masterhead masterhead  masterhead

Single-Cell Level Continuous Observation System for Microorganism Galvanotaxis


A novel system for measurement of motile microorganism galvanotaxis using high-speed vision is presented. Our goal is to construct a smart microsystem composed of many controlled microorganisms. The system utilizes galvanotaxis (intrinsic reaction to electrical stimulus) of microorganisms for actuation. For evaluation of taxis, continuous observation of a moving single cell in a sufficiently large working area without fixation is needed. Using high-speed vision, we developed a system for continuous evaluation of galvanotaxis of freely swimming cells in a large area at the single-cell level. Experimental results demonstrate the continuous measurement of galvanotaxis of a Paramecium caudatum cell moving in a 3.5-mm-square area for 18 s with 1 ms precision.

system electric stimulus input device


  1. Naoko Ogawa, Hiromasa Oku, Koichi Hashimoto and Masatoshi Ishikawa. Single-Cell Level Continuous Observation of Microorganism Galvanotaxis using High-Speed Vision. 2004 IEEE International Symposium on Biomedical Imaging (ISBI 2004) (Arlington, VA, USA, 2004.4.18) / Proceedings, pp. 1331-1334, Apr. 2004. [PDF (982K)] *IEEE
*IEEE © 2004 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.

Corresponding author: Naoko Ogawa (Naoko_Ogawa)

Ishikawa Watanabe Laboratory, Department of Information Physics and Computing, Department of Creative Informatics,
Graduate School of Information Science and Technology, University of Tokyo
Ishikawa Watanabe Laboratory WWW admin:
Copyright © 2008 Ishikawa Watanabe Laboratory. All rights reserved.